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Microbial metaproteomics : from sample processing, data collection to data analysis
Wu Enhui, Qiao Liang* Department of Chemistry, Fudan University, Shanghai 200433, China Microorganisms are closely related to human diseases and health. How to understand the composition of microbial communities and their functions is a major issue that needs to be studied ur...Read more -
Ten common mistakes in the use of liquid mobile phases!
The mobile phase is equivalent to the liquid phase of blood , and there are various things to pay attention to during use. Among them, there are some “pitfalls” that must be paid attention to. 01. Measure the pH of the mobile phase after adding organic solvent If you mea...Read more -
Common bad habits in the laboratory, how many do you have ?
Bad habits during the experiment 1. When weighing or measuring samples, record the data on a scratch paper first, and then copy it into the notebook after the sample is done; sometimes the records are filled in uniformly after the experiment is completed; 2. For steps that require t...Read more -
The reagent solution is a “double-edged sword”, and the safety bottle cap builds protection
Reagent solvents are tools for laboratory workers and also a source of safety hazards. Laboratory status: 1. The use of a large amount of organic solvents causes solvent volatilization; 2. There are no safety protection measures, the smell is strong, and it affects the health of employees; 3. The...Read more -
The 17 most toxic laboratory reagents, don’t be careless!
DMSO DMSO is dimethyl sulfoxide, which has a wide range of uses. It is used as a solvent for acetylene, aromatic hydrocarbons, sulfur dioxide and other gases, as well as a solvent for acrylic fiber spinning. It is an extremely important non-protonic polar solvent that is soluble in both wa...Read more -
The Difference Between Nylon 6 and Nylon 66
What are Nylon 6 and Nylon 66 Nylon 6 and nylon 66 are the main products of nylon. Nylon is strong and wear-resistant, and its strength is even comparable to that of steel wire of the same thickness; 15% nylon mixed into wool can increase its wear resistance by 3.5 times; except for polypropylene...Read more -
Correct use and steps of volumetric flask
Volumetric flasks are mainly used to accurately prepare solutions of a certain concentration. They are a thin, pear-shaped, flat-bottomed glass bottle with a ground stopper. There is a mark on the neck of the bottle. When the liquid in the bottle reaches the mark at the specified temperature, its...Read more -
What is HLB SPE column
What is HLB SPE column Bond Elut HLB (Hydrophile-Lipophile Balance) is an efficient, versatile solid phase extraction (SPE) sorbent formulated from monodisperse divinylbenzene and N-vinylpyrrolidone copolymers in specific ratios. This advanced sorbent provides excellent retention of a wide range ...Read more -
GC Basics
1. Principle of gas chromatography Chromatography, also called layer analysis, is a physical separation technology. The principle of Ader separation is to distribute the components in the mixture between two phases. One phase is stationary and is called the stationary phase. The other phase is th...Read more -
GC Operation tips
1 heating Due to the different manufacturers and quality of gas chromatographs, the methods of setting the temperature are also different. For setting the temperature using the microcomputer setting method or the dial selection method, it is usually to directly set the number or select an appropr...Read more -
Different caps
There are three types of caps available for sample vials: crimp caps, bayonet caps, and screw caps. Each sealing method has its own advantages. 1. Crimp cap The crimp cap squeezes the septum between the rim of the glass vial and the crimped aluminum cap. The sealing effect is very good and effect...Read more -
Precautions for injection needles – Liquid phase
\1. When using a manual injector for injection, the injection syringe must be cleaned with a needle wash solution before and after injection. The needle wash solution is generally selected to be the same solvent as the sample solution. The injection syringe must be cleaned with the sample solutio...Read more
